2017-09-25 17:19:392025-04-06 06:47:35
locus
BSU05910
BSU_05910
geneLength
474
477
outlinks
bsu
BSU05910
BSU_05910
Gene
Phenotypes of a mutant
reduced growth rate [Pubmed|19246765]
reduced growth rate [Pubmed|19246765]
increased sensitivity to paraquat [pubmed|28890133]
The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([[protein|TsaB]]-[[protein|TsaD]]-[[protein|TsaE]]) [Pubmed|23072323,21183954]
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([[protein|TsaB]]-[[protein|TsaD]]-[[protein|TsaE]]) [Pubmed|23072323,21183954]
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
phosphorylation of [[protein|TsaD]] [pubmed|28890133]
The protein
Protein family
UPF0079 family (according to Swiss-Prot)
TsaE family (single member, according to UniProt)
The protein
Structure
[PDB|1HTW] [Pubmed|12112691] (from '' Haemophilus influenzae'', 40% identity)
[PDB|5MVR] [Pubmed|28890133]
Biological materials
Expression vector
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP833, available in [SW|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP841, available in [SW|Stülke] lab
References
The protein
Paralogous protein(s)
[[this]]
The protein
Modification
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 [pubmed|28890133]
The protein
[SW|Cofactors]
Mg2+ [pubmed|28890133]
Biological materials
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP833, available in [SW|Stülke] lab
for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP841, available in [SW|Stülke] lab